Venetoclax Sensitivity in IFN-γ Stimulated CD34+ Blasts
CD34+ blasts cultured in R20 media were stimulated with 10 ng/ml of human recombinant IFN-γ. Unstimulated (PBS) blasts were included for comparison. After 12 h of culture, blasts with or without IFN-γ stimulation were counted and plated in a U-bottom 96-well plate (5 × 104 per well), with or without 10 ng/ml of human recombinant IFN-γ, along with titrated concentrations of venetoclax [Cat# S8048, Selleck Chemicals]. After 24 h of incubation, cell viability was assessed using the CellTiter-Glo luminescent cell viability assay [Cat# G7572, Promega] following the manufacturer’s protocol. IC50 values were calculated using GraphPad Prism version 10.1.0.
Other organizations :
The University of Texas MD Anderson Cancer Center, Harbin Medical University, University of Houston, Albert Einstein College of Medicine
Stimulation with 10 ng/ml of human recombinant IFN-γ
Unstimulated (PBS) blasts for comparison
positive controls
Blasts with IFN-γ stimulation
negative controls
Unstimulated (PBS) blasts
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