EVs were fixed with 1% glutaraldehyde solution for 5 min and washed with distilled water thrice. The fixed EVs were centrifuged at 13,000 × g for 3 min, based on previous protocols (Jung et al., 2020 (link)). Then, the supernatant was removed. Next, the samples were dehydrated in absolute ethanol for 10 min and placed on formvar‐carbon‐coated copper grids (TED PELLA, Inc., Redding, CA, USA). The grids were contrasted with 1% phosphotungstic acid for 1 min and then washed with distilled water thrice. The grids were dried off completely and then examined with a JEM‐2100F field‐emission electron microscope (JEOL Ltd., Japan). For cryo‐TEM, an aliquot of concentrated EVs was applied to the Lacey carbon grid (Electron Microscopy Science, Hatfield, PA, USA). The grids were stored in liquid nitrogen, transferred to a cryo‐specimen holder, and maintained at −180°C. Images were collected at a magnification of ×18,000 to ×29,000 on the Tecnai F20 Twin TEM operated at 200 kV.