Transfection of PDA Cells with UTX and GATA siRNAs

The plasmid pCMV-HA-UTX was generated by Addgene. Small interfering RNAs (siRNAs) synthesized by Invitrogen were as follows: UTX siRNA (#1: 5′-gcaaauguuccaguguauagguuua-3'; stealth#2: 5′-ucaguuagcuuugguugacuguaau-3′) and GATA SiRNA (#1:5′-guggacucuacaugaaacutt-3’; #2, 5′-gcucugguaauagcaauaatt-3′). Negative control siRNA (Invitrogen) and control pCMV vectors were used. Plasmids and siRNAs were transfected into PDA cells using Lipofectamine 2000 CD transfection reagent (Invitrogen). For transient transfection, cells were transfected with plasmids or siRNA at different doses as indicated for 48 h before functional assays.²⁵ (link)^,42 (link)^,43 (link)