Extraction and Quantification of PCB52 and Metabolites

PCB52 and its hydroxylated metabolites were extracted from tissues,
serum, and cecum and intestinal content using a published protocol;^{40 (link),41 (link)} see the Supporting Information. Briefly,
PCB52 and its metabolites were extracted from homogenates by liquid–liquid
extraction and cleaned by solid-phase extraction with acidified silica
gel. PCB52 and metabolites were quantified in the multiple reaction
monitoring setting (MRM) on an Agilent 7890 A GC system equipped with
an Agilent 7000 Triple Quad and Agilent 7693 autosampler (Agilent
Technologies, Santa Clara, CA); see the Supporting Information.

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Bullert A.J., Li X., Gautam B., Wang H., Adamcakova-Dodd A., Wang K., Thorne P.S, & Lehmler H.J. (2024). Distribution of 2,2′,5,5′-Tetrachlorobiphenyl (PCB52) Metabolites in Adolescent Rats after Acute Nose-Only Inhalation Exposure. Environmental Science & Technology, 58(14), 6105-6116.

Publication 2024

Agilent 7890 A GC Agilent 7000 Triple Quad

Corresponding Organization : University of Iowa

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Variable analysis

independent variables

Extraction of PCB52 and its hydroxylated metabolites from tissues, serum, and cecum and intestinal content

dependent variables

Quantification of PCB52 and its metabolites in the multiple reaction monitoring setting (MRM) on an Agilent 7890 A GC system equipped with an Agilent 7000 Triple Quad and Agilent 7693 autosampler

control variables

Extraction method using liquid-liquid extraction and solid-phase extraction with acidified silica gel

controls

Not explicitly mentioned
Not explicitly mentioned

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