Diverse Pancreatic Cancer Cell Lines

The human pancreatic carcinoma cells Panc1 (CRL-1469) and MiaPaCa2 (CRL-1420) were purchased from the American Type Culture Collection (ATCC, Rockville, MD). Patient derived pancreatic ductal adenocarcinoma cells MCW512, corresponding to MCW-4 from our prior report, were obtained from the Medical College of Wisconsin Surgical Oncology Biobank using IRB approved protocols and cultured as previously published (10 (link)). The cell lines K8282 and K8484 were derived from the original KRas^LSL.G12D/+-p53^R172H/+-Pdx^Cre (KPC) mice on the mixed 129/SvJae/C57BL/6 background and were the kind gift of Dr. Kenneth Olive (Columbia University, NY). FC1199, FC1242, FC1245, and DT10022 cell lines were derived from KPC mice in which each of the founder mutant mice had been backcrossed to the C57BL/6 genetic background. KPC cells were maintained in high glucose DMEM with 10% (v/v) FBS (Life Technologies Inc., Grand Island, NY). The Pan-02 cell lines were provided by the National Cancer Institute Cell Repository (Bethesda, MD) and maintained in RPMI-1640 with 10% (v/v) FBS.