Negative staining room temperature transmission electron microscopy (TEM) was performed as previously described [64 (link)]. Briefly, 5 µL of liposome suspension was added to previously negative glow discharged holy carbon grids (Science Service, Munich, Germany) and incubated for 10 s. After removing the excess sample with filter paper, liposomes were stained twice with 5 µL of a half-saturated uranyl acetate solution and air-dried. Samples were then transferred into a JEOL 1400Plus TEM, and images were obtained at 100 kV acceleration voltage on a TVIPS F416 4 kx4 k camera (Tietz Video and Image Processing Systems, Munich, Germany).
Peñate Medina T., Gerle M., Humbert J., Chu H., Köpnick A.L., Barkmann R., Garamus V.M., Sanz B., Purcz N., Will O., Appold L., Damm T., Suojanen J., Arnold P., Lucius R., Willumeit-Römer R., Açil Y., Wiltfang J., Goya G.F., Glüer C.C, & Peñate Medina O. (2020). Lipid-Iron Nanoparticle with a Cell Stress Release Mechanism Combined with a Local Alternating Magnetic Field Enables Site-Activated Drug Release. Cancers, 12(12), 3767.
Other organizations :
Second Affiliated Hospital of Zhejiang University, Helmholtz-Zentrum Hereon, Instituto de Nanociencia y Materiales de Aragón, Universidad de Zaragoza, Helsinki University Hospital, Päijät-Hämeen Keskussairaala
Liposome morphology and structure as observed by transmission electron microscopy (TEM)
control variables
Negative glow discharged holy carbon grids
Uranyl acetate staining solution
JEOL 1400Plus TEM with 100 kV acceleration voltage
TVIPS F416 4 kx4 k camera
controls
None specified
None specified
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