Plasmid Transfection Protocol for Cellular Studies

The following plasmids have been used in the manuscript: human SATB1 (Origene), human GBA (Origene), human a-SYN (A53T mutant) (Addgene), and mt-Keima (Addgene). The alphasynuclein-A53T plasmid was a gift from David Rubinsztein (Addgene plasmid # 40823; http://n2t.net/addgene:40823; RRID:Addgene_40823) (Furlong et al., 2000 (link)); mt-mKeima was a gift from Richard Youle (Addgene plasmid # 131626; http://n2t.net/addgene:131626; RRID:Addgene_131626) (Vargas et al., 2019 (link)). mKeima-Red-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 56018; http://n2t.net/addgene:56018; RRID:Addgene_56018) Transfections have been performed with Lipofectamin 3000 (Thermofisher) according to the manufacturer’s protocol.

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Russo T., Kolisnyk B., Aswathy B., Wan Kim T., Martin J., Plessis-Belair J., Ni J., Pearson J.A., Park E.J., Sher R.B., Studer L, & Riessland M. (2023). The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons. bioRxiv.

Publication Preprint 2023

human SATB1 human GBA mt-Keima mt-mKeima Lipofectamin 3000

Human Mito Plasmid Transfections

Corresponding Organization : Stony Brook University

Other organizations : Rockefeller University, Memorial Sloan Kettering Cancer Center, Stony Brook School, Baylor College of Medicine

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Variable analysis

independent variables

Human SATB1 (Origene)
Human GBA (Origene)
Human a-SYN (A53T mutant) (Addgene)
Mt-Keima (Addgene)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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