Twenty-five male and 8 female TRAP2;Ai14 mice (12–18 weeks of age) were bred in house to C57BL6/J wild type mice to give littermates of TRAP2+/− or TRAP−/−, and Ai14+/+, Ai14+/− or Ai14−/− genotypes (Transnetyx; Cordova, TN); a subset of mice (16 males and 6 females, TRAP+/−;Ai14+) was used to assess the feasibility of genetically labeling psilocybin-activated neural populations. Animals were maintained in age- and sex-matched groups of 2–5 littermates and housed in an 12:12 light-dark cycled SPF facility and appeared to be in good health; male mice weighed 31.4 ± 0.7 g (mean ± SEM) and females weighed 23 ± 0.9 g. Home cages were sterile ventilated cages by Innovive (San Diego, CA) offering irradiated 1/8” corn cob bedding (The Andersons, Inc.) with ad libitum access to pre-filled acidified water bottles (Innovive) and irradiated 18% protein rodent diet (Teklad Global). All animal behavioral procedures comply with the ARRIVE guidelines (Sert et al., 2020 (link)), the Guide for the Care and Use of Laboratory Animals (Council, 2011), and were approved by the Stanford University Institutional Animal Care and Use Committee. The animal care and use program is fully accredited by AAALAC, International and holds an Assurance with OLAW.
Mapping Psilocybin-Activated Neural Ensembles
Twenty-five male and 8 female TRAP2;Ai14 mice (12–18 weeks of age) were bred in house to C57BL6/J wild type mice to give littermates of TRAP2+/− or TRAP−/−, and Ai14+/+, Ai14+/− or Ai14−/− genotypes (Transnetyx; Cordova, TN); a subset of mice (16 males and 6 females, TRAP+/−;Ai14+) was used to assess the feasibility of genetically labeling psilocybin-activated neural populations. Animals were maintained in age- and sex-matched groups of 2–5 littermates and housed in an 12:12 light-dark cycled SPF facility and appeared to be in good health; male mice weighed 31.4 ± 0.7 g (mean ± SEM) and females weighed 23 ± 0.9 g. Home cages were sterile ventilated cages by Innovive (San Diego, CA) offering irradiated 1/8” corn cob bedding (The Andersons, Inc.) with ad libitum access to pre-filled acidified water bottles (Innovive) and irradiated 18% protein rodent diet (Teklad Global). All animal behavioral procedures comply with the ARRIVE guidelines (Sert et al., 2020 (link)), the Guide for the Care and Use of Laboratory Animals (Council, 2011), and were approved by the Stanford University Institutional Animal Care and Use Committee. The animal care and use program is fully accredited by AAALAC, International and holds an Assurance with OLAW.
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Corresponding Organization : Stanford University
Other organizations : University of Pennsylvania, VA Palo Alto Health Care System, Mental Illness Research, Education and Clinical Centers
Variable analysis
- Psilocybin administration
- Brain-wide distribution of c-Fos
- Feasibility of controlling neural ensembles activated by psilocybin using the TRAP2 mouse line
- Sex (male and female mice)
- Age (12-18 weeks)
- Genotype (TRAP2+/-, TRAP2-/-, Ai14+/+, Ai14+/-, Ai14-/-)
- Housing conditions (age- and sex-matched groups of 2-5 littermates, 12:12 light-dark cycle, SPF facility)
- Diet (irradiated 18% protein rodent diet)
- Water (pre-filled acidified water bottles)
- None specified
- Mice naive to experiments and treatments prior to the TRAPing timepoint
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