Lysolecithin (LPC)-mediated demyelination was performed as described in Kosaraju et al. (2020) (link). Briefly, 1-month-old WT mice were injected with LPC (Sigma, L1381) (1 μL of 1% solution in 1x PBS) using a stereotactic apparatus at two sites: +1.0 mm AP, +1.0 mm ML, −2.2 mm DV and +0.7 mm AP, +1.0ML, −2.2 DV. Animals were allowed to recover for 14 days.
Free full text: Click here