Surgical Injury to Trigeminal Nerve

Mice were anesthetized with isoflurane inhalation. Surgery was performed under an Omano surgical microscope (OM2300S-V7, 7-40X). A 1–1.5 cm midline neck incision was used. The superficial tissues were bluntly dissected and lateralized with a mini retractor. The neck muscles were gently dissected to locate the mouse’s right auditory bulla and the auditory capsule on the right side of the head, which are the landmarks to locate the foramen lacerum. A prepared piece of Surgifoam (Ethicon) at approximately 1–1.5 mg was gently delivered into the foramen lacerum using curved forceps. The Surgifoam was positioned between the trigeminal nerve root and the cochlea bulla. After removing the retractor and replacing the tissues, the skin was closed with 6-0 nylon monofilament (Ethicon) sutures. Mice in the sham group underwent the same surgical procedure including neck shaving, skin incision, muscle dissection, and foramen lacerum exposure without nerve root compression. The duration of the surgery ranged from 8 to 12 minutes per mouse. Tamoxifen was intraperitoneally administrated to Fos-iCre-ERT2 (Fos^2A-iCreER-knockin) mice immediately after the FLIT procedure.

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Ding W., Fischer L., Chen Q., Li Z., Yang L., You Z., Hu K., Wu X., Zhou X., Chao W., Hu P., Dagnew T.M., Dubreuil D.M., Wang S., Xia S., Bao C., Zhu S., Chen L., Wang C., Wainger B., Jin P., Mao J., Feng G., Harnett M.T, & Shen S. (2023). Highly synchronized cortical circuit dynamics mediate spontaneous pain in mice. The Journal of Clinical Investigation, 133(5), e166408.

Publication 2023

Surgifoam 6-0 nylon monofilament

Auditory Capsule Cochlea Dissection Ert2 Forceps Head Inhalation Isoflurane Mice Microscope Muscle Neck Neck muscles Nerve root compression Nylon 6 Root Skin Surgery Sutures Tamoxifen Tissues Trigeminal nerve

Corresponding Organization :

Other organizations : Massachusetts General Hospital, Harvard University, Massachusetts Institute of Technology, McGovern Institute for Brain Research, The University of Texas MD Anderson Cancer Center, Tufts University, University of Maryland, Baltimore, Athinoula A. Martinos Center for Biomedical Imaging, First Affiliated Hospital Zhejiang University, Emory University

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Variable analysis

independent variables

Surgifoam (Ethicon) delivery into the foramen lacerum

dependent variables

Not explicitly mentioned

control variables

Mice anesthesia with isoflurane inhalation
Surgical procedure under an Omano surgical microscope (OM2300S-V7, 7-40X)
Neck incision, muscle dissection, and foramen lacerum exposure without nerve root compression (sham group)

controls

Sham group: Underwent the same surgical procedure including neck shaving, skin incision, muscle dissection, and foramen lacerum exposure without nerve root compression

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