Analyzing Atherosclerosis Biomarkers in CIA

We used the One-Way analysis of variance tool embedded in Partek Genomics Suite (Partek Inc.) to compare differences in variable distribution between lesion and no-lesion regions of CIA per lesion type and between lesion type. We used Prism 8 (GraphPad) to perform Pearson’s correlation analysis and identify circulating miRNAs expressed at baseline that associated with the extent of atherosclerosis measured after 2-year diet challenge. Statistical significance was tested with p < 0.05, and all tests were two-tailed. Multiple testing correction was performed using FDR q-value < 0.05.