Meristem Size Determination in Plant Epidermis

Since the end of the meristem for each cell file is not the same, the meristem size was determined in every cell file of the epidermis. To do so, the distance from the QC to the first cell in focus in the epidermis was measured as well as the length of all other cells in the file. The end of the meristem was considered as the first elongated cell^{71 (link)}. The relative position of each cell was calculated by normalization to the length of the RAM in each cell file. Thus, in this analysis, the cell marking the end of the meristem will be at position 1 and 0 corresponds to the QC. The relative position was calculated according to: $\mathrm{Relative}\mathrm{position}=\frac{\left(\mathrm{Cell}\mathrm{length}+\mathrm{Initial}\mathrm{distance}\mathrm{from}\mathrm{the}\mathrm{QC}\right)}{\left(\mathrm{Length}\mathrm{of}\mathrm{cell}\mathrm{determining}\mathrm{the}\mathrm{end}\mathrm{of}\mathrm{the}\mathrm{meristem}+\mathrm{Initial}\mathrm{distance}\mathrm{from}\mathrm{the}\mathrm{QC}\right)}$
Epidermal T-clones and cells undergoing mitosis were removed from the analysis.