Cell Culture Protocol for Multiple Cell Lines

HT-29, 293T, DLD-1, and HCT-8 cells were obtained from the American Type Culture Collection (ATCC) and cultured according to their recommendations. The 5E clone of MCF10A cells was cultured as described previously (119 (link)). AC16 cells (110 (link)) were purchased from M. Davidson (Columbia University) and cultured in Dulbecco’s modified Eagle’s medium/F-12 medium (Life Technologies) with 12.5% tetracycline-free fetal bovine serum (Clontech) and penicillin-streptomycin (Gibco).

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Chitforoushzadeh Z., Ye Z., Sheng Z., LaRue S., Fry R.C., Lauffenburger D.A, & Janes K.A. (2016). TNF-insulin crosstalk at the transcription factor GATA6 is revealed by a model that links signaling and transcriptomic data tensors. Science signaling, 9(431), ra59.

Publication 2016

DLD-1 Dulbecco’s modified Eagle’s medium/F-12 medium tetracycline-free fetal bovine serum penicillin-streptomycin

Cells Clone cells Cultured medium Eagle Fetal bovine serum Ht 29 Penicillin Streptomycin Tetracycline

Corresponding Organization : University of Virginia

Other organizations : University of North Carolina at Chapel Hill, Massachusetts Institute of Technology

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Variable analysis

independent variables

Cell lines used: HT-29, 293T, DLD-1, HCT-8, 5E clone of MCF10A, and AC16

dependent variables

Not explicitly mentioned

control variables

Culture conditions for each cell line as recommended by the providers (ATCC, M. Davidson)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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