An in vitro immersion test calculating the weight loss after 72 h immersion under cell culture conditions (37 °C, 5% CO2, 20% O2, humidified atmosphere) was performed as described in Nidadavolu [53 (link)] in order to determine the impact of liposomes on the degradation of the Mg-10Gd material. Material discs 1.5 mm in height and 1 cm in diameter were cleaned utilizing 20 min sonication in n-hexane (Merck, Darmstadt, Germany), 20 min sonication in acetone (Merck, Darmstadt, Germany), and 3 min sonication in 100% ethanol. Lastly, specimens were sterilized and dried in 70% ethanol under sterile conditions [54 (link),55 (link)].
The discs were placed in Dulbecco’s modified eagle’s medium (DMEM, Life Technologies, Darmstadt, Germany) + 10% FBS (fetal bovine serum, PAA Laboratories, Linz, Austria): αMSH-SM-liposome (25:1 v:v), and in DMEM and DMEM 10% FCS: PBS (phosphate-buffered saline) (25:1 v:v). The Mg concentration released into the immersion medium was measured utilizing atomic absorption spectrometry (Agilent 240 AA, Agilent, CA, USA), as described [54 (link)]. The immersion assay was also performed with pure Mg using the same methodology.
The discs were placed in Dulbecco’s modified eagle’s medium (DMEM, Life Technologies, Darmstadt, Germany) + 10% FBS (fetal bovine serum, PAA Laboratories, Linz, Austria): αMSH-SM-liposome (25:1 v:v), and in DMEM and DMEM 10% FCS: PBS (phosphate-buffered saline) (25:1 v:v). The Mg concentration released into the immersion medium was measured utilizing atomic absorption spectrometry (Agilent 240 AA, Agilent, CA, USA), as described [54 (link)]. The immersion assay was also performed with pure Mg using the same methodology.
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