Protocol detail

Cellular Apoptosis Assays

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WM-3835, all the antibodies, viral constructs, and mRNA primers were provided by Dr. Cao at Soochow University [25 (link)]. The caspase inhibitors zDEVD-fmk and zVAD-fmk, as well as the cell counting kit-8 (CCK-8) kit, docetaxel, and lactate dehydrogenase (LDH) assay kit were purchased from Sigma (St. Louis, MO). All fluorescence dyes were purchased from Thermo-Fisher Invitrogen (Shanghai, China).

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Mi Y.Y., Ji Y., Zhang L., Sun C.Y., Wei B.B., Yang D.J., Wan H.Y., Qi X.W., Wu S, & Zhu L.J. (2023). A first-in-class HBO1 inhibitor WM-3835 inhibits castration-resistant prostate cancer cell growth in vitro and in vivo. Cell Death & Disease, 14(1), 67.

Publication 2023

zDEVD-fmk zVAD-fmk docetaxel fluorescence dyes

Antibodies Assay Caspase inhibitors Docetaxel Fluorescence dyes Lactate dehydrogenase Mrna Primers Zvad fmk

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Variable analysis

independent variables

Caspase inhibitors zDEVD-fmk and zVAD-fmk
Docetaxel

dependent variables

Cell viability (measured by CCK-8 kit)
Cell death (measured by LDH assay)

control variables

Antibodies, viral constructs, and mRNA primers provided by Dr. Cao at Soochow University
Fluorescence dyes purchased from Thermo-Fisher Invitrogen (Shanghai, China)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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