In situ Hi-C protocol for chromatin structure analysis
In situ Hi-C experiments were performed according to Concia et al.3 (link) using DpnII enzyme (New England Biolabs). DNA libraries were prepared using NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions (10 cycles for the PCR amplification step). DNA libraries were checked for quality and quantified using a 2100 Bioanalyzer (Agilent) and the libraries were subjected to 2 × 75 bp high-throughput sequencing by NextSeq 500 (Illumina). Two independent biological replicates were generated.
Corresponding Organization : Université Paris Cité
Other organizations :
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Université Paris-Saclay, University of Warwick, Instituto de Agrobiotecnología del Litoral, National University of the Littoral, Southwest University of Science and Technology, Biologie du Fruit et Pathologie, Université de Bordeaux, Inserm, Université Clermont Auvergne, Clermont Université, Institut National Polytechnique de Toulouse, Genomics and Biotechnology of the Fruits Laboratory, Fudan University, Zhejiang University, Goethe University Frankfurt, King Abdullah University of Science and Technology
NEBNext Ultra II DNA library preparation kit (NEB)
2 × 75 bp high-throughput sequencing by NextSeq 500 (Illumina)
Two independent biological replicates
controls
Positive control: Not specified
Negative control: Not specified
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