Protein Immunoblotting Procedure

Aliquots of the cell extracts were run on polyacrylamide-SDS gels, transferred to nitrocellulose membranes and blotted as described previously (5 (link),7 (link),8 (link)). The signals were detected using an ECL detection reagent (Thermo Fisher Scientific, 34077, 34095).

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Gadad S.S., Camacho C.V., Malladi V., Hutti C.R., Nagari A, & Kraus W.L. (2021). Poly(ADP-ribose) Polymerase-1 Regulates Estrogen-Dependent Gene Expression in Estrogen Receptor α-Positive Breast Cancer Cells. Molecular cancer research : MCR, 19(10), 1688-1698.

Publication 2021

ECL detection reagent

Cell extracts Membranes Nitrocellulose Polyacrylamide gels

Corresponding Organization : Texas Tech University Health Sciences Center

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Variable analysis

independent variables

Aliquots of the cell extracts

dependent variables

Signals detected using an ECL detection reagent

control variables

Polyacrylamide-SDS gels
Nitrocellulose membranes

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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