Quantitative BDNF and IGFBP-2 ELISA

Serum quantitative determination of BDNF was performed by ELISA using the Human Free BDNF Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions. The detection range was 62.5–4000 pg/mL, and the sensitivity was 20 pg/mL. Serum insulin-like growth factor binding protein-2 (IGFBP-2) concentration was determined by ELISA. The measurement of all other analytes reported here was described in previous papers [17 (link),18 (link)].

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Fachim H.A., Malipatil N., Siddals K., Donn R., Cortés G.Y., Dalton C.F., Gibson J.M, & Heald A.H. (2022). Methylation Status of Exon IV of the Brain-Derived Neurotrophic Factor (BDNF)-Encoding Gene in Patients with Non-Diabetic Hyperglycaemia (NDH) before and after a Lifestyle Intervention. Epigenomes, 6(1), 7.

Publication 2022

Human Free BDNF Quantikine ELISA Kit

Elisa Human bdnf Insulin like growth factor binding protein 2 Sensitivity Serum

Corresponding Organization : Manchester Academic Health Science Centre

Other organizations : Sheffield Hallam University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum quantitative determination of BDNF
Serum insulin-like growth factor binding protein-2 (IGFBP-2) concentration

control variables

None explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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