Bacterial strains, plasmids, and oligonucleotides used in this work are listed in Table 1 and Table 2 . The forty-three PJI S. aureus strains used in this study were isolated from patients admitted at Sahlgrenska University Hospital (Mölndal, Sweden) with PJI of the hip or knee [32 (link),33 ]. On the other hand, twenty-six strains from wound swabs, isolated between 1966 and 2010, were obtained from the Culture Collection University of Gothenburg (CCGU).
Escherichia coli and S. aureus strains were routinely grown in Luria-Bertani medium (LB; Conda-Pronadisa, Madrid, Spain) and Trypticase soy broth (TSB; Conda-Pronadisa, Madrid, Spain), respectively, at 37 °C. When required, media were supplemented with appropriate antibiotics at the following concentrations: erythromycin (Ery), 10 µg/mL or 2.5 µg/mL; ampicillin (Amp), 100 µg/mL. Bacteriological agar was used as a gelling agent (VWR, Radnor, PA, USA).
Escherichia coli and S. aureus strains were routinely grown in Luria-Bertani medium (LB; Conda-Pronadisa, Madrid, Spain) and Trypticase soy broth (TSB; Conda-Pronadisa, Madrid, Spain), respectively, at 37 °C. When required, media were supplemented with appropriate antibiotics at the following concentrations: erythromycin (Ery), 10 µg/mL or 2.5 µg/mL; ampicillin (Amp), 100 µg/mL. Bacteriological agar was used as a gelling agent (VWR, Radnor, PA, USA).
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