An anatomic specimen embalmed by means of the Anubifix™ method was used. This embalming technique is based on a new prerinsing method combined with a normal 4% formaldehyde fixation solution. In contrast to conventional embalming methods, Anubifix™ embalming results in a very small decrease in flexibility and plasticity. Furthermore, this result is accomplished without impairing the quality and duration of conservation. Anubifix™ embalming results in a preserved range of motion of joints and flexibility of the abdominal wall combined with tissue tactility comparable to fresh-frozen tissues, this all in contrast to conventional embalming methods.
After the embalming phase, a midline laparotomy of 20 cm was performed. The specific aspects of the colorectal anatomy were dissected and marked [aorta and iliac arteries (dark red), superior mesenteric artery/vein and its branches (red/blue), inferior mesenteric artery/vein and its branches (red/blue), gonadal arteries (purple), and ureters (yellow)]. Coloring of the vessels and ureters was performed circumferentially with a specially developed formaldehyde-proof paint (FPP™). After dissection and coloring, the abdominal muscle wall was separated from the overlying fat and skin and closed with running sutures. A rectangular sheet of synthetic butyl rubber measuring 26 × 6 cm with a circular hole at the level of the umbilicus was sutured on top of the sutured muscle wall, analogous to an onlay mesh for incisional hernia, after which the skin was closed with running sutures. The use of a butyl rubber sheet results in an airtight closure of the abdominal wall permitting the creation of a pneumoperitoneum despite the prior abdominal opening. A 10-mm trocar was placed at the umbilicus through which a 30º scope was placed. A standard set of laparoscopic instruments was used. Four 5-mm trocars were placed in the right and left upper quadrants. Pneumoperitoneum was achieved with a continuous flow of CO2, with the pressure set between 12 and 15 mmHg, i.e., comparable to the in vivo situation.
After the embalming phase, a midline laparotomy of 20 cm was performed. The specific aspects of the colorectal anatomy were dissected and marked [aorta and iliac arteries (dark red), superior mesenteric artery/vein and its branches (red/blue), inferior mesenteric artery/vein and its branches (red/blue), gonadal arteries (purple), and ureters (yellow)]. Coloring of the vessels and ureters was performed circumferentially with a specially developed formaldehyde-proof paint (FPP™). After dissection and coloring, the abdominal muscle wall was separated from the overlying fat and skin and closed with running sutures. A rectangular sheet of synthetic butyl rubber measuring 26 × 6 cm with a circular hole at the level of the umbilicus was sutured on top of the sutured muscle wall, analogous to an onlay mesh for incisional hernia, after which the skin was closed with running sutures. The use of a butyl rubber sheet results in an airtight closure of the abdominal wall permitting the creation of a pneumoperitoneum despite the prior abdominal opening. A 10-mm trocar was placed at the umbilicus through which a 30º scope was placed. A standard set of laparoscopic instruments was used. Four 5-mm trocars were placed in the right and left upper quadrants. Pneumoperitoneum was achieved with a continuous flow of CO2, with the pressure set between 12 and 15 mmHg, i.e., comparable to the in vivo situation.
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