Simulating Microgravity Effects on Hodgkin's Lymphoma

Human Hodgkin’s lymphoma (HL) cell lines L-540 and HDLM-2 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany)^{71 (link)}. The cell lines were maintained in RPMI 1640 (Life Technologies, USA) supplemented with 10% fetal bovine serum (FBS, Life Technologies, USA) and 1% penicillin/streptomycin solution (Life Technologies, USA) at 37 °C in 5% CO₂. For cellular ROS inhibition, the cells were added with N-acetylcysteine (NAC, Sigma Aldrich, USA) at 10 mM in complete medium for operating clinostat. Simulation of microgravity using clinostat were previously described^{46 (link)}. All experiments were performed after human HL cells were cultured under 1 G or taSMG condition for 2 days.