Protocol detail

Culturing and Isolating C. albicans Strains

Find Similar Protocols

All strains of C. albicans were cultured at 37°C with aeration in 5 ml of YPD [yeast extract (10 g/liter; VWR J850-500G), peptone (20 g/liter; VWR J636-500G), and dextrose (20 g/liter; VWR BDH9230-500G)] broth. For in vivo mouse experiments, C. albicans strains were grown static overnight in 10 ml of YPD. The selection of the urinary clinical strains tested in this study was based on high fungal burden during isolation (~10⁶ to 10⁸ CFU/ml) and infections developed at different time points and urinary tract sites. Pt62 was recovered from a patient that had a catheter for 1 day. Pt65 was recovered from a patient that had a catheter for 6 days. PCNL1 is a kidney stone clinical isolate from a catheterized patient with a C. albicans kidney infection.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

La Bella A.A., Andersen M.J., Gervais N.C., Molina J.J., Molesan A., Stuckey P.V., Wensing L., Nobile C.J., Shapiro R.S., Santiago-Tirado F.H, & Flores-Mireles A.L. (2023). The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection. Science Advances, 9(9), eade7689.

Publication 2023

C albicans Catheter Dextrose Infections Isolation Kidney infection Kidney stone Mouse Patient Peptone Strains Urinary Urinary tract Yeast

Top 5 similar protocols

Variable analysis

independent variables

Strains of C. albicans

dependent variables

Fungal burden during isolation (approximately 10^6 to 10^8 CFU/ml)
Time point of infection development
Urinary tract site of infection

control variables

Culture conditions (37°C with aeration in 5 ml of YPD broth)
Overnight static growth in 10 ml of YPD (for in vivo mouse experiments)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!