Apoptosis and Cell Viability Assays

Apoptosis and cell viability studies were performed using the Caspase-Glo and CellTiter-Glo luminescence-based assays (Promega) [41 (link)]. Cells were plated in 96-well flat-bottomed plates (Perkin Elmer) after transfection and incubated for 48 hours (Caspase-Glo) or 72 hours (CellTiter-Glo). Luminescence was measured with a BioTek Synergy 2 Luminometer (BioTek). Data were normalized to the cells transfected with non-targeting control siRNA.

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Rausch J.L., Boichuk S., Ali A.A., Patil S.S., Lee D.M., Brown M.F., Makielski K.R., Liu Y., Taguchi T., Kuan S.F, & Duensing A. (2016). Opposing roles of KIT and ABL1 in the therapeutic response of gastrointestinal stromal tumor (GIST) cells to imatinib mesylate. Oncotarget, 8(3), 4471-4483.

Publication 2016

Caspase-Glo CellTiter-Glo 96-well flat-bottomed plates BioTek Synergy 2 Luminometer

Apoptosis Caspase Cell viability Cells Luminescence Luminescence assays Promega Sirna Transfection

Corresponding Organization : University of Pittsburgh

Other organizations : Kazan State Medical University, Kōchi University

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Variable analysis

independent variables

Transfection

dependent variables

Apoptosis (measured by Caspase-Glo assay)
Cell viability (measured by CellTiter-Glo assay)

control variables

Cell plating in 96-well flat-bottomed plates
Incubation time (48 hours for Caspase-Glo, 72 hours for CellTiter-Glo)
Luminescence measurement using BioTek Synergy 2 Luminometer

controls

Cells transfected with non-targeting control siRNA (used for data normalization)

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