Ang II-induced hypertension in mice

For induction of hypertension, 8-week-old male mice were administered Ang II (1.4 mg/kg/d, Sigma-Aldrich, St. Louis, MO) continuously for 14 days via a subcutaneous osmotic minipump (Alzet Model 100.2, DURECT Corporation), which was implanted at the right side back of mice with a minor surgical procedure in the isoflurane-anesthetized animals. A portion of mice also received i.p. injection of Src inhibitor SU6656 (8 mg/kg/d, Sigma-Aldrich, St. Louis, MO) during the last 2 days (day 13 and 14) of Ang II treatment. Arterial systolic, diastolic, and mean BP were measured by the standard noninvasive tail-cuff method (CODA System, Kent Scientific, Torrington, Conn) as we described previously [14 (link)]. Ang II solution was prepared by dissolving 13 mg Ang II in 2.21 mL PBS. SU6656 was prepared by dissolving 25 mg SU6656 in 3.52 mL DMSO, followed by dilution with 5.4 mL to final concentration.