Apomorphine-Induced Turning Behavior Assay

To test apomorphine-induced turning behavior the animals received in the neck a subcutaneous injection of 0,05 mg/kg apomorphine hydrochloride (Sigma) dissolved in 1% ascorbic acid 0,9% NaCl, and placed on metal testing bowls (MED-RSS, Med Associates) for 45 minutes. The number of contralateral rotations was digitally recorded, and 6-OHDA-lesioned animals presenting more than 100 rotations in 45 minutes were considered to have a nearly complete lesion (n = 28), whereas the ones presenting less than 100 rotations were classified as having an incomplete lesion (n = 13). Sham animals (n = 19) showed no turning behavior.

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Carvalho M.M., Campos F.L., Coimbra B., Pêgo J.M., Rodrigues C., Lima R., Rodrigues A.J., Sousa N, & Salgado A.J. (2013). Behavioral characterization of the 6-hydroxidopamine model of Parkinson’s disease and pharmacological rescuing of non-motor deficits. Molecular Neurodegeneration, 8, 14.

Publication 2013

6 ohda Animals Apomorphine Apomorphine hydrochloride Ascorbic acid Metal Nacl Neck Subcutaneous injection

Corresponding Organization :

Other organizations : University of Minho

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Variable analysis

independent variables

Apomorphine hydrochloride dose (0.05 mg/kg)

dependent variables

Number of contralateral rotations

control variables

Injection location (subcutaneous in the neck)
Injection solution (apomorphine hydrochloride dissolved in 1% ascorbic acid 0.9% NaCl)
Testing environment (metal testing bowls)
Testing duration (45 minutes)

controls

6-OHDA-lesioned animals presenting more than 100 rotations in 45 minutes (considered to have a nearly complete lesion)
Sham animals showing no turning behavior

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