Transcriptomic Profiling of Idiopathic PAH Lungs

Idiopathic PAH lung tissue and human control lung tissue were used for global profiles. The analyses for total RNA in lung tissue were performed as formerly outlined [4 (link)]. Shortly, biotinylated cRNA was prepared from 6 ug total RNA (Expression Analysis). 10 ug of cRNA was hybridized at 45C for a time period of 16 hours on GeneChip Genome Array. The data were determined with Partek Genomics Suite 6.6 using Affymetrix default analysis settings and global scaling as the normalization method. Using Partek Genomics Suite 6.6, the value definition was set up and using a t-test with a false discovery rate (FDR) of 2 fold, P<0.01 was determined. The database has been submitted to NCBI/GEO and has been approved and assigned a GEO accession number, GSE53408.