The monomers used were 1, 3,
5-triazine-2, 4, 6-triamine (melamine, 99%) from Merck, Darmstadt,
Germany, and polyoxymethylene (paraformaldehyde, extra pure) from
BDH, Poole, UK. Acetonitrile (ACN, p.a.), formic acid (FA, 98–100%),
phosphoryl trichloride (POCl3; 99%), α-hydro-ω-hydroxy-poly[oxy(1-methylethylene)]
[PPG4000; poly(propylene oxide), 4000 Da], iodoacetamide (IAA), 1,
4-dithiothreitol (DTT), triethylammonium bicarbonate (TEAB) buffer
(1 M), deuterium oxide (D2O, 99.9% atom-% D), and deuterated
acetonitrile (CD3CN, >99.8 atom-% D) were from Merck.
Trifluoroacetic
acid (TFA) was a product of VWR Chemicals (Radnor, PA, USA). The methanol
used in the Soxhlet extraction was of analytical grade from Prolabo,
obtained from VWR. Tetrahydrofuran (THF, 99.5% anhydrous, < 0.005%
H2O dried over molecular sieves, stabilized with 2, 6-di-tert-butyl-4-methylphenol) was from Scharlau (Barcelona,
Spain). Phenylphosphoric acid (PPA) was a product of TCI (Geel, Belgium).
The amphiphilic triblock copolymers Pluronic L61, L121, P123, and
F127, α, ω-hydroxy-poly(oxyethylene)-block-poly[oxy(1-methylethylene)]-block-poly(oxyethylene)
of varying overall molecular weights and block ratios were gifts from
BASF (Ludwigshafen, Germany). Dialysis tubing (MWCO 1000 Da) was from
Spectrum Laboratories (New Brunswick, NJ, USA). The GADDYYTAR peptides
(non- and mono-phosphorylated on tyrosine) were custom-synthesized
by LifeTein (Somerset, NJ, USA), and trypsin was from Promega (Madison,
WI, USA). All chemicals were used as received, unless otherwise noted.
Water used was prepared by Milli-Q or Ultra-Q equipment from Merck
Millipore (Bedford, MA, USA).
5-triazine-2, 4, 6-triamine (melamine, 99%) from Merck, Darmstadt,
Germany, and polyoxymethylene (paraformaldehyde, extra pure) from
BDH, Poole, UK. Acetonitrile (ACN, p.a.), formic acid (FA, 98–100%),
phosphoryl trichloride (POCl3; 99%), α-hydro-ω-hydroxy-poly[oxy(1-methylethylene)]
[PPG4000; poly(propylene oxide), 4000 Da], iodoacetamide (IAA), 1,
4-dithiothreitol (DTT), triethylammonium bicarbonate (TEAB) buffer
(1 M), deuterium oxide (D2O, 99.9% atom-% D), and deuterated
acetonitrile (CD3CN, >99.8 atom-% D) were from Merck.
Trifluoroacetic
acid (TFA) was a product of VWR Chemicals (Radnor, PA, USA). The methanol
used in the Soxhlet extraction was of analytical grade from Prolabo,
obtained from VWR. Tetrahydrofuran (THF, 99.5% anhydrous, < 0.005%
H2O dried over molecular sieves, stabilized with 2, 6-di-tert-butyl-4-methylphenol) was from Scharlau (Barcelona,
Spain). Phenylphosphoric acid (PPA) was a product of TCI (Geel, Belgium).
The amphiphilic triblock copolymers Pluronic L61, L121, P123, and
F127, α, ω-hydroxy-poly(oxyethylene)-block-poly[oxy(1-methylethylene)]-block-poly(oxyethylene)
of varying overall molecular weights and block ratios were gifts from
BASF (Ludwigshafen, Germany). Dialysis tubing (MWCO 1000 Da) was from
Spectrum Laboratories (New Brunswick, NJ, USA). The GADDYYTAR peptides
(non- and mono-phosphorylated on tyrosine) were custom-synthesized
by LifeTein (Somerset, NJ, USA), and trypsin was from Promega (Madison,
WI, USA). All chemicals were used as received, unless otherwise noted.
Water used was prepared by Milli-Q or Ultra-Q equipment from Merck
Millipore (Bedford, MA, USA).
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