Exosome Isolation from Peripheral Blood

Peripheral venous blood samples were obtained in the morning after performing overnight PSG (PSG was conducted after the coronary angiography surgery in patients with AMI). All of blood samples (8 mL from peripheral venous blood) were collected using a conventional blood collection tube (usually a red head), and stored at room temperature for 2 h until a significant clot is produced. After centrifuged at 1200 g for 10 min at 4 °C and 1800 g for 10 min at 4 °C, the supernatants were collected and frozen at -80 °C until use. As ultracentrifugation can accurately and repeatedly obtain exosomes while minimizing co-purification of protein aggregates and other membrane particles, in our study exosomes were isolated by ultracentrifugation and the quality was determined with an electron microscope [20 (link)].