MTLn3 were cultured and transfected as previously described 10 (link).
MDA-MB-231 (ATCC HTB-26), BT-549 (ATCC HTB-122) were cultured in DMEM with 10% fetal bovine serum (FBS), antibiotics (Invitrogen, Grand Island, NY) and insulin (for BT-549) (Invitorgen) as suggested by ATCC.
MCF10A (ATCC CRL 10317) were cultured following the ATCC protocols. MCF10A were transfected as previously described23 (link). MDA-MD-231 and BT-549 were transfected by nucleofection according to manufacturer's protocols (Lonza, Basel, Switzerland). All cell lines mycoplasma tested.
MDA-MB-231 (ATCC HTB-26), BT-549 (ATCC HTB-122) were cultured in DMEM with 10% fetal bovine serum (FBS), antibiotics (Invitrogen, Grand Island, NY) and insulin (for BT-549) (Invitorgen) as suggested by ATCC.
MCF10A (ATCC CRL 10317) were cultured following the ATCC protocols. MCF10A were transfected as previously described23 (link). MDA-MD-231 and BT-549 were transfected by nucleofection according to manufacturer's protocols (Lonza, Basel, Switzerland). All cell lines mycoplasma tested.
