Established Canine Melanoma Cell Lines

Four established canine melanoma cell lines were used: CML-1, CML-6 M, and CML-10c2 (originally described by Lauren Wolfe et al. from Auburn University), and 17CM98 from the University of Wisconsin. All cell lines were generously provided by Mike Huelsmeyer from the University of Wisconsin, Madison, Wisconsin. CML-1, CML-6 M, CML-10c2, and 17CM98 originated from an oral melanoma, a lymph node metastasis from a primary cutaneous melanoma, a primary cutaneous melanoma, and a lymph node metastasis from a primary oral melanoma, respectively [44 (link), 45 (link)]. Cells were grown in monolayer culture in Dulbecco’s Modified Eagle Medium (DMEM) (Wisent, St-Bruno, Quebec) with 10% fetal bovine serum (FBS) (Wisent, St-Bruno, Quebec), 100 U/mL penicillin/streptomycin and 2.50 μg/mL amphotericin-B (Thermo Fisher Scientific, Waltham, Massachusetts) added to the media. Cell cultures were kept in a controlled environment at 37 °C humidified air and 5% CO₂.