Cell Line Maintenance and Characterization
Corresponding Organization : Hong Kong Science and Technology Parks Corporation
Other organizations : Hainan Medical University, Queen Mary Hospital, Memorial Sloan Kettering Cancer Center, Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, University of Georgia, Wuhan Institute of Virology
Variable analysis
- Cell lines obtained from the American Type Culture Collection: Calu3, Caco2, 293T, Huh7, and VeroE6
- 293T-ACE2 obtained from GeneCopoeia
- A549-ACE2 obtained from Invivogen
- VeroE6-TMPRSS2 obtained from the Japanese Collection of Research Bioresources Cell Bank
- Not explicitly mentioned
- Culture medium: Dulbecco's modified Eagle's medium (DMEM) or DMEM/F12 according to supplier's instructions
- All cell lines are routinely tested for and maintained as mycoplasma-free
- No positive or negative controls were explicitly mentioned
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