Calu3, Caco2, 293T, Huh7, and VeroE6 were obtained from the American Type Culture Collection. 293T-ACE2 was obtained from GeneCopoeia (Rockville, Maryland, USA). A549-ACE2 was obtained from Invivogen (San Diego, CA, USA). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Amarillo, Texas, USA) or DMEM/F12 (Gibco) according to the supplier’s instructions (47 (link)). VeroE6-TMPRSS2 was obtained from the Japanese Collection of Research Bioresources Cell Bank and cultured in DMEM. All cell lines used are routinely tested for mycoplasma and are maintained mycoplasma-free.
Corresponding Organization : Hong Kong Science and Technology Parks Corporation
Other organizations :
Hainan Medical University, Queen Mary Hospital, Memorial Sloan Kettering Cancer Center, Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, University of Georgia, Wuhan Institute of Virology
Cell lines obtained from the American Type Culture Collection: Calu3, Caco2, 293T, Huh7, and VeroE6
293T-ACE2 obtained from GeneCopoeia
A549-ACE2 obtained from Invivogen
VeroE6-TMPRSS2 obtained from the Japanese Collection of Research Bioresources Cell Bank
dependent variables
Not explicitly mentioned
control variables
Culture medium: Dulbecco's modified Eagle's medium (DMEM) or DMEM/F12 according to supplier's instructions
All cell lines are routinely tested for and maintained as mycoplasma-free
controls
No positive or negative controls were explicitly mentioned
Annotations
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