The fixed slices were treated with 3% H2O2 and then blocked with 5% BSA. The specimens were finally incubated with anti-Filaggrin (Santa Cruz, California, United States) or anti-Involucrin (1: 500; Absin, Shanghai, China) antibody overnight at 4°C, and then incubated with horseradish peroxidase-conjugated secondary antibody at 37°C for 4 h followed by reaction with 3,3-diaminobenzidine (DAB).
Free full text: Click here