Protocol detail

Drosophila Genetic Toolkit Protocols

Find Similar Protocols

Flies were maintained using standard conditions. Fly lines repo-Gal4 (BDSC_7415), longGMR-Gal4 (BDSC_8121), elav-Gal4 (BDSC_8760), Hdc^MB07212 (BDSC_25260), R10D10-Gal4 (BDSC_69558), R32H04-Gal4 (BDSC_49734), R29A12-Gal4 (BDSC_49478), and R19C02-Gal4 (BDSC_49282) were provided by the Bloomington Drosophila Stock Center. The mCD8-GFP and 3XPax3-GFP markers and the carT^43A mutant have been described^{25 (link)}. UAS-WNK-RNAi and UAS-fray-RNAi transgenes and their effectiveness in knocking down the relevant target have been described^{10 (link)}. The UAS-Ncc69-RNAi line (VDRC KK106499) was obtained from the Vienna Drosophila Resource Center (VDRC). The Ncc69^r1, Ncc69^r2 and Gli-Gal4; UAS-Ncc69 flies^{13 (link)} were a gift from Dr. William Leiserson (Yale University, New Haven, CT). The MZ709-Gal4 line was a gift from Dr. Hong-Sheng Li (University of Massachusetts Medical Center, Worcester, MA).

Free full text: Click here

Stenesen D., Moehlman A.T., Schellinger J.N., Rodan A.R, & Krämer H. (2019). The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system. Scientific Reports, 9, 2475.

Publication 2019

repo-Gal4 longGMR-Gal4 elav-Gal4

Drosophila Rnai Standard Transgenes

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Knockdown of WNK, fray, and Ncc69 genes using RNAi
Expression of Ncc69 gene using Gli-Gal4; UAS-Ncc69 flies

dependent variables

Not explicitly mentioned

control variables

Fly lines maintained using standard conditions
MCD8-GFP and 3XPax3-GFP markers used
CarT43A mutant used

controls

UAS-WNK-RNAi and UAS-fray-RNAi transgenes used as knockdown controls
Ncc69r1, Ncc69r2, and Gli-Gal4; UAS-Ncc69 flies used as controls

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!