SDS-PAGE and Western Blotting for Protein Expression

Cells were lysed and the resulting supernatant was loaded in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel as described previously [11 (link)]. The antibodies used were anti-SMYD1 (Abcam, 1:1,000), anti-SRF (Santa Cruz, 1:2,000), and anti-GFP (Abcam, 1:1,000). Specific IRDye® 800CW conjugated goat (polyclonal) anti-rabbit IgG was used to detect protein expression with an Odyssey infrared imaging system (LI-COR Bioscience, U.S.A.).

Free full text: Click here

Ye X., Qian Y., Wang Q., Yuan W., Mo X., Li Y., Jiang Z., Xu W., Deng Y., Wan Y., Fan X., Wu X, & Wang Y. (2016). SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis. PLoS ONE, 11(1), e0146468.

Publication 2016

anti-SRF anti-GFP Odyssey infrared imaging system

Anti igg Antibodies Cells Goat Irdye 800cw Protein Rabbit Sds page

Corresponding Organization : East China Normal University

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein expression

control variables

Cells were lysed and the resulting supernatant was loaded in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel as described previously [11 (link)]
The antibodies used were anti-SMYD1 (Abcam, 1:1,000), anti-SRF (Santa Cruz, 1:2,000), and anti-GFP (Abcam, 1:1,000)
Specific IRDye® 800CW conjugated goat (polyclonal) anti-rabbit IgG was used to detect protein expression with an Odyssey infrared imaging system (LI-COR Bioscience, U.S.A.)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!