Anesthetic Exposure on Cell Cultures

When cultures reached to 60%, they were exposed to the experimental gas mixture which consisted of 21% O₂, 5% CO₂ and either 3.6% sevoflurane or 10.3% desflurane balanced with N₂ (BOC, South Humberside, UK) in a purpose-built 1.5 L airtight gas chamber, equipped with inlet and outlet valves. The chamber was placed in an incubator (Galaxy R CO₂ chamber; New Brunswick Scientific, Enfield, CT, USA) at 37 °C for 2 h [53 (link)]. Other cohort cells were exposed to the same concentration gases without inhalational anesthetic served as controls. After exposure, cells were returned to the normal culture incubator until the further study.