All samples were retrieved from archives of the University of California, Davis Alzheimer’s Disease Center (UCD-ADC) Brain Bank. Archival samples analyzed in this study were 5 μm formalin fixed, paraffin embedded, sections of the superior and middle temporal gyrus. The tissue had been previously stained with an Aβ antibody (4G8, recognizing residues 17–24, dilution 1:1600, BioLegend (formally Covance), catalog number SIG-39200) that were first pretreated with formic acid to rid samples of endogenous protein. All slides were digitized using an Aperio AT2 up to ×40 magnification. Supplementary Table 1 details overall NIA Reagan criteria65 , CAA type66 (link) 1 or 2 within the section, and Thal Amyloid phase5 (link) of these cases.
Procedures were in accordance with ethical standards of the Helsinki Declaration. Operations of the University of California Davis Alzheimer’s Disease Center was approved by the Institutional Review Board (IRB) of the University of California Davis, and written consent for autopsy was obtained for each participant during life. Details of this program have been previously published67 (link).
Procedures were in accordance with ethical standards of the Helsinki Declaration. Operations of the University of California Davis Alzheimer’s Disease Center was approved by the Institutional Review Board (IRB) of the University of California Davis, and written consent for autopsy was obtained for each participant during life. Details of this program have been previously published67 (link).
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