Renal Histopathological Evaluation

Renal sections (3 µm thick) fixed in Duboscq-Brasil and embedded in paraffin were stained with periodic acid-Schiff (PAS) reagent (Bio-Optica, Milan, Italy) and assessed by light microscopy. Tubular lesions, evaluated as tubular dilation and cytoplasmic vacuolation in tubule segments and collecting ducts, were examined in 15–20 fields for each animal and were expressed with a score from 0 to 3 (mild, moderate and severe). For renal ultrastructural analysis, glutaraldehyde-fixed fragments of cortical kidney tissue were processed as previously described [33 (link)] and examined with a Philips Morgagni 268D transmission electron microscope (TEM; Philips, Brno, Czech Republic).

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Buelli S., Locatelli M., Carminati C.E., Corna D., Cerullo D., Imberti B., Perico L., Brigotti M., Abbate M., Zoja C., Benigni A., Remuzzi G, & Morigi M. (2022). Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome. Cells, 11(11), 1755.

Publication 2022

PAS) reagent Morgagni 268D

Animal Cortical of kidney Cytoplasmic Dilation Embedded paraffin Glutaraldehyde Light microscopy Periodic acid Renal Schiff reagent Transmission electron microscope

Corresponding Organization : Mario Negri Institute for Pharmacological Research

Other organizations : University of Bologna

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Variable analysis

independent variables

Renal sections (3 µm thick) fixed in Duboscq-Brasil and embedded in paraffin

dependent variables

Tubular lesions, evaluated as tubular dilation and cytoplasmic vacuolation in tubule segments and collecting ducts
Renal ultrastructural analysis

control variables

Sections were stained with periodic acid-Schiff (PAS) reagent (Bio-Optica, Milan, Italy)
Tubular lesions were examined in 15–20 fields for each animal
Glutaraldehyde-fixed fragments of cortical kidney tissue were processed as previously described [33 (link)]
Renal ultrastructural analysis was performed with a Philips Morgagni 268D transmission electron microscope (TEM; Philips, Brno, Czech Republic)

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