Flow Cytometric Analysis of EMT Markers
Corresponding Organization : Translational Research Institute for Metabolism and Diabetes
Variable analysis
- Staining for membrane surface molecules
- Intracellular staining of CCT2
- Expression of EpCAM-PE
- Expression of E-cadherin-PE
- Expression of N-cadherin-APC
- Expression of CCT2-PE
- Use of isotype controls for EpCAM-PE, E-cadherin-PE, N-cadherin-APC, and CCT2-PE
- Isotype control [MOPC-21] for EpCAM-PE and CCT2-PE
- Isotype control [A95-1] for E-cadherin-PE
- Isotype control [27–35] for N-cadherin-APC
- Isotype control [MOPC-21] for CCT2-PE
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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