Serum creatinine concentration was used to calculate eGFR according to the CKD Epidemiology Collaboration equation.17 (link) Serum creatinine concentration was calibrated to isotope dilution using mass spectrometry. Urine albumin concentration was measured with the BNII ProSpec (Siemens, Inc., Munich, Germany). Urine creatinine concentration was measured by the Jaffe method on the Modular P chemistry analyzer (Roche/Hitachi, Basel, Switzerland). We adjusted for urine albumin and urine creatinine concentrations separately in multivariable models, whereas albuminuria was expressed as UACR in descriptive statistics.18 (link)
Sociodemographics and aspects of medical history were self-reported at the baseline interview. Prevalent cardiovascular disease was defined as self-reported stroke, myocardial infarction, coronary artery bypass graft, angioplasty, arterial stenting, or as evidence of past myocardial infarction on electrocardiography. Blood pressure was defined as the average of 2 measures taken on seated participants after a 5-minute rest. Use of medications for hypertension was obtained by self-report. Body mass index was determined using measured height and weight.
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