H9c2 cell viability was determined using MTT assay according to the manufacturer’s instructions (Wang B. et al., 2015 (link)). After OGD treatment, MTT solution was added to the wells and further incubated for 4 h at 37°C, then solubilized with 100 μL DMSO. The absorbance was measured at 490 nm using a microplate reader (Thermo, New York, NY, United States). Cell viability was calculated as follows: Viability (%) = (OD of Assay – OD of Blank)/(OD of control -OD of blank) × 100%.
To evaluate the degree of cell injury induced by OGD, LDH released into culture media were measured by LDH activity assay kit (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.
To evaluate the degree of cell injury induced by OGD, LDH released into culture media were measured by LDH activity assay kit (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.
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