Immunofluorescence Microscopy Reagents

Rabbit polyclonal antibodies (pAbs) for ERC1, FLAG, FN, LL5β (recognizing human and monkey LL5β, but not mouse LL5β), and mouse monoclonal antibodies (mAbs) for ERC1a (clone ELKS-30), tubulin, FLAG clone M2 (Sigma-Aldrich); rabbit pAb for GFP (Life Technologies); chicken pAb for GFP and rabbit pAb (Abcam); rabbit pAb anti DS-RED (Clontech); rabbit pAb for Liprin-α1 (Protein Tech); mouse mAb for filamin A (Millipore), paxillin (clone 349, BD Biosciences), GST (Amersham Biosciences), His-tag (Qiagen), LL5α/β (clone 1H12) [19 (link)]; hamster mAb for LL5α [5 (link)] was kindly provided by dr. Yuko Mimori-Kiyosue (RIKEN Center for Biosystems Dynamics Research, Kobe, Japan). Secondary Abs Alexa-488, Alexa-568, Alexa-546 and Alexa-647, phalloidin Alexa-568 and Alexa-647, and Oregon green 488-gelatin (Life Technologies); HRP-conjugated anti-rabbit and anti-mouse secondary Abs (Jackson and Amersham Biosciences). Other reagents included: FN (Corning); poly-L-lysine hydrobromide.

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Ribolla L.M., Sala K., Tonoli D., Ramella M., Bracaglia L., Bonomo I., Gonnelli L., Lamarca A., Brindisi M., Pierattelli R., Provenzani A, & de Curtis I. (2023). Interfering with the ERC1–LL5β interaction disrupts plasma membrane–Associated platforms and affects tumor cell motility. PLOS ONE, 18(7), e0287670.

Publication 2023

His-tag Alexa-488 Alexa-546 Alexa-568 Oregon green 488-gelatin

Alexa 568 Anti ds Antibodies Chicken Clone Elks Filamin a Gelatin Hamster Human Lysine Monkey Mouse Oregon green 488 Paxillin Phalloidin Poly Protein Rabbit Tubulin

Corresponding Organization : Vita-Salute San Raffaele University

Other organizations : University of Florence, University of Trento

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal antibodies (pAbs) for ERC1, FLAG, FN, LL5β (recognizing human and monkey LL5β, but not mouse LL5β)
Mouse monoclonal antibodies (mAbs) for ERC1a (clone ELKS-30), tubulin, FLAG clone M2 (Sigma-Aldrich)
Rabbit pAb for GFP (Life Technologies)
Chicken pAb for GFP
Rabbit pAb anti DS-RED (Clontech)
Rabbit pAb for Liprin-α1 (Protein Tech)
Mouse mAb for filamin A (Millipore), paxillin (clone 349, BD Biosciences), GST (Amersham Biosciences), His-tag (Qiagen), LL5α/β (clone 1H12) [19]
Hamster mAb for LL5α [5]

dependent variables

Not explicitly mentioned

control variables

Secondary Abs Alexa-488, Alexa-568, Alexa-546 and Alexa-647
Phalloidin Alexa-568 and Alexa-647
Oregon green 488-gelatin (Life Technologies)
HRP-conjugated anti-rabbit and anti-mouse secondary Abs (Jackson and Amersham Biosciences)
FN (Corning)
Poly-L-lysine hydrobromide

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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