Antibody Sources for Protein Analysis

The rabbit anti-human PAD2 and PAD4 antibodies were obtained from Proteintech (12110–1-AP; Rosemont, IL) and Sigma-Aldrich (P4749; St. Louis, MO), respectively. The rabbit anti-human caspase-3, activating transcription factor 4 (ATF4), 78 kDa Glucose-regulated Protein (GRP78) and beta actin antibodies were obtained from Abcam (ab4051; Cambridge, UK), Santa Cruz Biotechnology (sc-200; Dallas, TX), LS Bio (LS-C312961; Seattle, WA), and Abcam (ab8227), respectively. The mouse anti-dog/cat estrogen receptor alpha (ERα) antibody was obtained from Thermo Fisher Scientific (MA5–13065; Waltham, MA). The human anti-modified citrulline antibody was obtained from Millipore (MABS487; Darmstadt, Germany). Alexa 488-conjugated goat anti-rabbit antibodies, HRP-conjugated goat anti-mouse antibodies, HRP-conjugated goat anti-rabbit and HRP-conjugated goat anti-human antibodies were all obtained from Jackson ImmunoResearch Labs (West Grove, PA). The PAD inhibitor, BB-CLA, was synthesized as previously described and diluted in dimethyl sulfoxide (DMSO) [25 (link)].

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Ledet M.M., Anderson R., Harman R., Muth A., Thompson P.R., Coonrod S.A, & Van de Walle G.R. (2018). BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway. BMC Cancer, 18, 412.

Publication 2018

ab8227 MA5–13065 HRP-conjugated goat anti-mouse antibodies HRP-conjugated goat anti-rabbit HRP-conjugated goat anti-human antibodies

Activating transcription factor 4 Anti antibodies Anti antibody Antibodies Antibody Beta actin Caspase 3 Citrulline Dimethyl sulfoxide Goat Grp78 Human Mouse Rabbit

Corresponding Organization :

Other organizations : Cornell University, University of Massachusetts Chan Medical School

Top 5 similar protocols

Variable analysis

independent variables

PAD inhibitor, BB-CLA

dependent variables

PAD2 protein levels
PAD4 protein levels
Caspase-3 protein levels
ATF4 protein levels
GRP78 protein levels
Citrullinated protein levels

control variables

Beta actin protein levels
Estrogen receptor alpha (ERα) protein levels

positive controls

Rabbit anti-human PAD2 and PAD4 antibodies
Rabbit anti-human caspase-3, ATF4, GRP78 antibodies
Mouse anti-dog/cat estrogen receptor alpha (ERα) antibody
Human anti-modified citrulline antibody

negative controls

Alexa 488-conjugated goat anti-rabbit antibodies
HRP-conjugated goat anti-mouse antibodies
HRP-conjugated goat anti-rabbit antibodies
HRP-conjugated goat anti-human antibodies

Annotations

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