Isolation and Characterization of Dip G

Dip G (>98% purity) was isolated as previously described^{22 (link)}. Chloroquine, oil red O, sodium butyrate (NaB), methyl thiazolyl tetrazolium (MTT) and tamoxifen (TAM) were purchased from Sigma-Aldrich (St. Louis, MO). Z-VAD-FMK and necrostatin-2 were from Selleck (Houston, TX). Anti-E-cadherin (14472), anti-vimentin (5741), anti-N-cadherin(13116), anti-p-β-catenin (Ser33/37/Thr41) (9561), anti-p-β-catenin (Thr41/Ser45) (9565), anti-p-β-catenin (Ser552) (5651), anti-p-β-catenin (Ser675) (4176), anti-β-catenin (8480), anti-c-Myc (18583), anti-LC3B (3868) and anti-GABARAPL1 (26632) were purchased from Cell Signaling Technology (Beverly, MA). Polyclonal anti-GABARAPL1 (11010-1-AP) was from Proteintech Group (Chicago, IL). Anti-ERα (sc-8002), anti-ERβ (sc-373853 X) and anti-β-Actin (sc-47778) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-SQSTM1/p62 (ab101266) was from Abcam (Cambridge, UK). CD24 monoclonal antibody (FITC) (11-0247-42), CD44 monoclonal antibody (PE-eFluor 610) (61-0441-80), Lipofectamine 3000 and Lipofectamine RNAi MAX were purchased from Thermo Fisher Scientific (Waltham, MA).

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Fan M., Chen J., Gao J., Xue W., Wang Y., Li W., Zhou L., Li X., Jiang C., Sun Y., Wu X., Wu X., Ge H., Shen Y, & Xu Q. (2020). Triggering a switch from basal- to luminal-like breast cancer subtype by the small-molecule diptoindonesin G via induction of GABARAPL1. Cell Death & Disease, 11(8), 635.

Publication 2020

Chloroquine oil red O tamoxifen Z-VAD-FMK Anti-E-cadherin ), anti-vimentin anti-N-cadherin anti-p-β-catenin anti-p-β-catenin (Ser552) anti-GABARAPL1 anti-β-Actin (sc-47778) Anti-SQSTM1/p62 (ab101266) Lipofectamine 3000 Lipofectamine RNAi MAX

Actin Cadherin Cd44 Chloroquine Fitc Lipofectamine Monoclonal antibody Oil red o Rnai Sodium butyrate Tamoxifen Tetrazolium Vimentin Z vad fmk β catenin

Corresponding Organization :

Other organizations : Nanjing University, Nanjing Medical University

Top 5 similar protocols

Variable analysis

independent variables

Dip G (>98% purity)
Chloroquine
Oil red O
Sodium butyrate (NaB)
Methyl thiazolyl tetrazolium (MTT)
Tamoxifen (TAM)
Z-VAD-FMK
Necrostatin-2

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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