Hyperpolarized 13C Metabolite Preparation

Unless otherwise indicated, all chemicals and solvents were purchased from Sigma-Aldrich (St. Louis, MO). 13C metabolites were prepared for HP according to published reports: [1-13C] pyruvate24 (link), [1-13C] lactate35 (link), [13C,15N2] urea36 (link), [1-13C] dehydroascorbate and [1-13C] ascorbate21 (link). All metabolites were HP using a prototype SpinLab (General Electric, Niskayuna, New York, USA) for approximately 90 min before dissolving with appropriate buffers as described in previous publications.

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Tee S.S., DiGialleonardo V., Eskandari R., Jeong S., Granlund K.L., Miloushev V., Poot A.J., Truong S., Alvarez J.A., Aldeborgh H.N, & Keshari K.R. (2016). Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnetic Field. Scientific Reports, 6, 32846.

Publication 2016

SpinLab

Buffers Electric Solvents

Corresponding Organization :

Other organizations : Memorial Sloan Kettering Cancer Center, Hunter College, Cornell University

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Variable analysis

independent variables

Preparation of 13C metabolites: [1-13C] pyruvate, [1-13C] lactate, [13C,15N2] urea, [1-13C] dehydroascorbate, and [1-13C] ascorbate

dependent variables

Not explicitly mentioned

control variables

Chemical and solvent sources: Sigma-Aldrich (St. Louis, MO)
Hyperpolarization (HP) of 13C metabolites: Performed using a prototype SpinLab (General Electric, Niskayuna, New York, USA) for approximately 90 min before dissolving with appropriate buffers as described in previous publications

controls

Positive controls: Not specified
Negative controls: Not specified

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