Validating DECR Expression in Clinical Samples

Expression of identified DECRs was validated further in clinical samples by RT-qPCR. RNA isolation and RT-qPCR were undertaken as described previously (Zhou et al., 2021 (link)). In brief, red blood cell (RBC) lysis buffer (catalog number: C3702; Beyotime Institute of Biotechnology, Shanghai, China) was used to split RBCs. Subsequent extraction of total RNA by TRIzol® Reagent and synthesis of complementary-DNA were carried out according to manufacturer (Takara Biotechnology, Shiga, Japan) protocols. mRNA expression was quantified using the ABI-9700 system (Applied Biosystems, Foster City, CA, United States) and normalized against the housekeeping gene β-actin by the comparative quantification method (2^−ΔΔCT). The primers used in this study are listed in Supplementary Table S2.