The aptamer-mediated cell binding was carried out as previously reported [20 (link)]. One hour after incubation with indicated aptamers at 200 nM concentration in wells of a 24-well plate, 1 µL of Hoechst 33342 solution (1 µg/mL) was added to the plated cells and incubated for 10min at 37 °C in a humidified incubator supplying 5% CO2/air. The cells were then washed prior to visualization using a Nikon Eclipse TS100 Inverted Fluorescence Microscope system (Nikon).
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