Bimolecular Fluorescence Complementation of Oncogenes

Human cDNAs of c‐Myc (1365 bp), LMNA (1995 bp) and LMNC (1719 bp) were inserted into pBiFC‐VN173 (containing Flag‐tag) or pBiFC‐VC155 (containing HA‐tag) from Addgene. The c‐Myc and LMNA constructs were mutated by QuikChange Multi Site‐Directed Mutagenesis Kit (Agilent) using primer sets (Table S5). Constructs were co‐transfected into tumour cells by using NeuroPorter Transfection Kit (Sigma) for a 24‐h period, while fluorescence imaging was detected using a confocal microscope.
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Wang J., Hong M., Cheng Y., Wang X., Li D., Chen G., Bao B., Song J., Du X., Yang C., Zheng L, & Tong Q. (2024). Targeting c‐Myc transactivation by LMNA inhibits tRNA processing essential for malate‐aspartate shuttle and tumour progression. Clinical and Translational Medicine, 14(5), e1680.

Publication 2024

pBiFC‐VN173 pBiFC‐VC155 QuikChange Multi Site‐Directed Mutagenesis Kit NeuroPorter Transfection Kit

Corresponding Organization : Huazhong University of Science and Technology

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Variable analysis

independent variables

Mutations in c-Myc and LMNA constructs

dependent variables

Fluorescence imaging of co-transfected tumor cells

control variables

Plasmid constructs (pBiFC-VN173 and pBiFC-VC155)
Transfection method (NeuroPorter Transfection Kit)
Transfection duration (24-h period)

controls

Positive control: None specified
Negative control: None specified

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