For the colony-formation assays in monolayer cultures, LLC and SPC-A1 cells (150 cells/well) were seeded in 100 mm dishes for 24 h and then treated with TAX at different concentrations for 10 days. Then, the cells were fixed in 4% paraformaldehyde (Servicebio, Wuhan, China) at 4 °C and stained with crystal violet (Biosharp, Hefei, China). Details can be found in reference [17 (link)]. Further details are also shown in the Supplementary Materials.
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