For the colony-formation assays in monolayer cultures, LLC and SPC-A1 cells (150 cells/well) were seeded in 100 mm dishes for 24 h and then treated with TAX at different concentrations for 10 days. Then, the cells were fixed in 4% paraformaldehyde (Servicebio, Wuhan, China) at 4 °C and stained with crystal violet (Biosharp, Hefei, China). Details can be found in reference [17 (link)]. Further details are also shown in the Supplementary Materials.
Lu J., Shuai B., Shou Z., Guo W., Zhou C., Ouyang X., Zhou H., Li J., Cui J., Jiang F., Jin K.Y., Sarapultsev A., Li F., Zhang G., Luo S, & Hu D. (2022). Taraxasterol Inhibits Tumor Growth by Inducing Apoptosis and Modulating the Tumor Microenvironment in Non-Small Cell Lung Cancer. Cancers, 14(19), 4645.
Corresponding Organization : Ministry of Education of the People's Republic of China
Other organizations :
Wuhan Children's Hospital, Integrated Chinese Medicine (China), Tianjin University of Traditional Chinese Medicine, Xiamen University Malaysia, South Ural State University, Hong Kong Baptist University
LLC and SPC-A1 cells (150 cells/well) seeded in 100 mm dishes for 24 h
controls
No specific positive or negative controls were mentioned in the provided information.
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