A colony of A. maculatum was developed from wild-caught adults and maintained as previously described (Troughton and Levin 2007 (link)). Briefly, nymphal and adult ticks were fed on adult Sprague-Dawley rats (Division of Laboratory Animal Medicine, Louisiana State University) within capsules fashioned from 50-ml plastic conical tubes and attached with a 3:1 rosin to beeswax mixture. Engorged females were kept in vials at 27°C and ≈90% RH. Larvae were fed on adult BALB/c mice (Louisiana State University Division of Laboratory Animal Medicine) housed on wire grates over fresh water, and engorged larvae were collected twice daily as the water was changed. For this experiment, 120 nymphs, which originated from the same egg clutch were used. This colony of ticks is constitutively infected with rickettsiae, and sequence analysis using standard PCR for rompA (Rickettsia outer membrane protein A) identified this organism as the nonpathogenic Candidatus “Rickettsia andeanae” (Paddock et al. 2010 (link)).
Protocol detail
Rearing of Amblyomma maculatum ticks
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Adult
Balb c mice
Beeswax
Capsules
Females
Laboratory animal
Larvae
Medicine
Nymphs
Rickettsia
Rickettsia outer membrane protein a
Rosin
Sequence analysis
Sprague dawley rats
Ticks
Corresponding Organization : Louisiana State University
Other organizations : Mississippi State University, Centers for Disease Control and Prevention, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Top 5 similar protocols
Protocol cited in 7 other protocols
Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- Temperature (27°C)
- Relative humidity (≈90% RH)
- Species of host animals used for feeding (adult Sprague-Dawley rats for nymphal and adult ticks, adult BALB/c mice for larvae)
- Origin of tick colony (wild-caught adults)
- Positive control: None mentioned
- Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!