Glucose concentration was measured every 10 min during the insulin clamp with an Analox glucose analyzer (Analox Instruments, London, U.K.). [6,6-2H2]-d -glucose enrichment in the plasma and infusate was measured using gas chromatography–mass spectrometry. As described previously, the steady-state equations of Steele et al. (29 (link)) were used to calculate the rate of glucose appearance (Ra) and disappearance (Rd). EGP was calculated as the difference between total glucose Ra and the exogenous glucose infusion rate, peripheral insulin sensitivity was assessed from the rate of glucose infusion required to maintain euglycemia during the high-dose insulin clamp, and hepatic insulin sensitivity was assessed by the extent to which EGP was suppressed from baseline to low-dose hyperinsulinemia (26 (link)).
Protocol detail
Insulin Clamp and Glucose Kinetics
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Euglycemia clamp
Gas chromatography mass spectrometry
Glucose
Hyperinsulinemia
Insulin
Insulin sensitivity
Plasma
Corresponding Organization : Mayo Clinic
Other organizations : Howard Hughes Medical Institute, Yale University
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Protocol cited in 1 other protocol
Variable analysis
independent variables
- Insulin clamp
- Glucose concentration
- [6,6-2H2]-d-glucose enrichment in the plasma and infusate
- Rate of glucose appearance (Ra)
- Rate of glucose disappearance (Rd)
- Endogenous glucose production (EGP)
- Peripheral insulin sensitivity
- Hepatic insulin sensitivity
- Analox glucose analyzer (Analox Instruments, London, U.K.) used to measure glucose concentration
- Gas chromatography–mass spectrometry used to measure [6,6-2H2]-d-glucose enrichment in the plasma and infusate
- Steady-state equations of Steele et al. (29) used to calculate Ra and Rd
- EGP calculated as the difference between total glucose Ra and the exogenous glucose infusion rate
- Peripheral insulin sensitivity assessed from the rate of glucose infusion required to maintain euglycemia during the high-dose insulin clamp
- Hepatic insulin sensitivity assessed by the extent to which EGP was suppressed from baseline to low-dose hyperinsulinemia
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